Colwyn Bay and Guilsfield of the Cymru North and Cwmbran Celtic and Ammanford in the Cymru South were drawn as the clubs receiving byes in to the second round[2] The draw for the second round took place on 25 July and was conducted by Cardiff City assistant manager Tom Ramasut.
The 12 JD Cymru Premier clubs enter the competition at the second round stage and are joined by Barry Town United and Cefn Druids, who were relegated from the top-flight last season.
[3] On 28 July 2022, Llandudno, Abergavenny Town and Briton Ferry Llansawel were removed from the Nathaniel MG Cup for rule breaches.
[4] The draw for the 2022/23 Nathaniel MG Cup third round took place at 8:15pm on Thursday 11 August and was conducted by Sgorio's Mike Davies.
Flint Town United, despite losing in the previous round were reinstated due to a competition rule breach by Colwyn Bay.