Originally anchored by Mel Tiangco and Mike Enriquez, it premiered on March 15, 2004, on the network's Telebabad line up.

24 Oras premiered on March 15, 2004, broadcasting from Studio 2 of GMA Network Center, replacing Frontpage: Ulat ni Mel Tiangco.

[4] On August 24, 2018, newscaster Atom Araullo served as a substitute anchor for Enriquez, who went on a medical leave.

[9] On June 1, 2020, Enriquez, Tiangco and Cruz returned to anchor from the studio set of the program.

[10][11] On October 11, 2021, host Kim Atienza joined the show to anchor the segment #KuyaKimAnoNa.