Located at the intersection of 25th Street and Fourth Avenue in Greenwood Heights, Brooklyn, it is served by the R train at all times.

The station opened on June 22, 1915, as part of the initial portion of the BMT Fourth Avenue Line to 59th Street.

[5] The 25th Street station was constructed as part of the Fourth Avenue Line, the plan for which was initially adopted on June 1, 1905.

[12][13] Though the Transit Commission ordered the BMT to lengthen these platforms in September 1923, no further progress was made until February 16, 1925, when the New York City Board of Transportation (NYCBOT) commissioned its engineers to examine platform-lengthening plans for this and eleven other stations along the Fourth Avenue Line.

[19] In the 1960s, the NYCTA started a project to lengthen station platforms on its lines in Southern Brooklyn to 615 feet (187 m) to accommodate 10-car trains.

[5] On July 14, 1967, the NYCTA awarded a contract to conduct test borings at eleven stations on the Fourth Avenue Line, including 25th Street, to the W. M. Walsh Corporation for $6,585 (equivalent to $60,172 in 2023) in preparation of the construction of platform extensions.

The NYCTA spokesman stated that the old tiles were in poor condition and that the change was made to improve the appearance of stations and provide uniformity.

[30][7]: 854  The walls were intended to improve ventilation, as passing trains would push air forward, rather than to the sides of the tunnel.

[7] However, the original trim line is still visible in the fare control areas behind the token booth and MetroCard Vending Machines.

[37][38] The entrance dates back to 1862,[39] and is a New York City designated landmark, made of New Jersey brownstone with sculpted groups.