28S ribosomal RNA

It has a size of 25S in plants and 28S in mammals, hence the alias of 25S–28S rRNA.

[1] Combined with 5.8S rRNA to the 5' side, it is the eukaryotic nuclear homologue of the prokaryotic 23S and mitochondrial 16S ribosomal RNAs.

The comparison of the sequences from these genes are sometimes used in molecular analysis to construct phylogenetic trees, for example in protists,[5] fungi,[6] insects,[7] arachnids,[8] tardigrades,[9] and vertebrates.

[12] Some eukaryotes cleave 28S rRNA into two parts before assembling both into the ribosome, a phenomenon termed the "hidden break".

[12] Several databases provide alignments and annotations of LSU rRNA sequences for comparative purposes:

Mitochondrial 16S and nuclear 28S ribosomal DNA phylogenies of three species of mollusks belonging to the genus Waldo .