Upon being mustered into Confederate service, the regiment served in the Savannah defenses, Military District of Georgia.

Some of the early battles in the East that the regiment took part in included South Mountain, Sharpsburg, Chancellorsville, and Gettysburg.

The regiment went south when James Longstreet took his corps to Georgia and Tennessee in the fall of 1863, where it was engaged at the Siege of Knoxville.

This regiment, formed in March 1862 served with the Army of Northern Virginia from July 1862 until its surrender at Appomattox Court House, except during Longstreet's 1863 expedition to Georgia and Tennessee.

Toomb's Georgia troops were credited with defending the bridge over Antietam creek against several ill-fated attempts to cross it, until finally being pushed back late in the afternoon.