The regiment was attached to 2nd Brigade, 4th Division, Army of the Tennessee, to July 1862.
2nd Brigade, 4th Division, District of Memphis, Tennessee, to September 1862.
2nd Brigade, 4th Division, District of Jackson, Tennessee, to November 1862.
3rd Brigade, 4th Division, XVI Corps, to July 1863.
The 53rd Indiana Infantry mustered out July 21, 1865, at Louisville, Kentucky.