Acromyrmex insinuator will produce a minor working caste that tends to the fungal garden, which is unique in comparison to the other known social parasites in the Attini tribe.

[3] However, a more recent study has noted a decrease in the metapleural glands of the A. insinuator workers in comparison to their host.

[citation needed] While the impact A. insinuator have on their host is not well documented, it makes sense to state that they have a negative effect on the fitness of A. echinatior.

[citation needed] Winged queen ants and males leave their respective colonies in large groups in a flight known as the revoada ("Flock" in English).

Each female mates with multiple males to collect the 300 million sperm she needs to set up a colony.

[7] With large appetites and the inability to forage for themselves, the larvae are completely reliant on the adult colony to provide them with food.

[9] Eventually the pupae emerge in their fully-grown adult forms and depending on the amount of food they received and their sex, they will either be designated as a Queen, Female or Male worker.

[9] While A. insinuator specifically has no direct impact on humans, leaf-cutter ants overall are a significant problem in many areas of the world, but also vital in their ecosystems.

[10] The large size of the ant colonies (30-600m2) cause many issues ranging from crop devastation to reducing land surfaces useless as the ground beneath is unstable.

Also the effect Wolbachia bacteria strains that are unique to A. insinuator may affect the species in comparison to their close relatives A.