It has one platform and is located in the network administered by the Verkehrsverbund Rhein-Neckar (VRN).

[1] It was opened with the Odenwald Railway (Odenwaldbahn) from Heidelberg to Würzburg as a through station on 23 October 1862.

Südwestdeutsche Verkehrs-Aktiengesellschaft (SWEG) took over the line from Deutsche Bundesbahn under a fixed 20-year lease on 1 January 1982.

With the transfer of responsibility for the Elsenz Valley Railway and the Schwarzbach Valley Railway to the Rhine-Neckar S-Bahn, the line was electrified, Aglasterhausen station was completely modernised and a raised platform was installed there.

The station is served by line S 51 of the Rhine-Neckar S-Bahn every hour from 5am to midnight, Monday to Friday, with extra trains in the peaks.