[1] Alessandro Sperelli was born on May 5, 1589 in Assisi, Italy and ordained a priest in the Congregation of the Oratory of Saint Philip Neri.

[4][2] On 4 May 1642, he was consecrated bishop by Giulio Cesare Sacchetti, Cardinal-Priest of Santa Susanna, with Lelio Falconieri, Titular Archbishop of Thebae, and Giovanni Battista Altieri (seniore), Bishop Emeritus of Camerino, serving as co-consecrators.

[2] On 14 Mar 1644, he was appointed during the papacy of Pope Urban VIII as Bishop of Gubbio.

[1][2] While bishop, he was the principal co-consecrator of Carlo Labia, Archbishop of Corfù (1659).

[2] In 1666, his endowment of his large collection of books and manuscripts to the city led to the formation of the Biblioteca Comunale Sperelliana in Gubbio.