Her first husband was Hendrik van Brederode, who played an important part in the events leading up to the Eighty Years' War.
After his death in 1568, she married Frederick III, Elector Palatine of the Rhine in 1569.
It was in the same year that Emilia, the second daughter of William the Silent and his second wife Anna of Saxony was named after her.
In 1590 she was given the rights of use of Alpen, Helpenstein, Lennep and Erbvogtei of Köln by her half-sister, Magdalena.
Alpen was occupied by the Dutch Republic in 1597 and the following year by Spanish forces.