Ambrose is a city in Coffee County, Georgia, United States.

As of the 2020 census, the city had a population of 327.

Ambrose is located at 31°35′38″N 83°0′57″W / 31.59389°N 83.01583°W / 31.59389; -83.01583 (31.594015, -83.015819).

[4] According to the United States Census Bureau, the city has a total area of 3.1 square miles (8.0 km2), of which 3.1 square miles (8.0 km2) is land and 0.1 square miles (0.26 km2) (1.92%) is water.

In 2020, its population was 327, down from 380 in 2010.