The Amory Lock is located in the city of Amory, Mississippi, and the waterway forms the west and north boundary of the city.

Mississippi Highway 6 crosses the waterway at an overpass south of the Amory Lock.

The Amory Lock is located at mile 371.1 on the waterway, representing the navigational distance from the southern end of the waterway at the Cochrane–Africatown USA Bridge on U.S. Route 90 in Mobile, Alabama.

[2] All locks on the waterway use marine radio channel 16 for standby; the Amory Lock's unique marine radio channel is 14.

For large vessels, lock operation is made on demand.