Amphistereum is a genus of fungi in the family Auriculariaceae.

[1] Species produce cupulate to effused, leathery basidiocarps (fruit bodies) on wood.

Microscopically, fruit bodies have a dimitic hyphal system.

The genus is currently only known from North and South America.

[2] The genus was created as a result of molecular research, based on cladistic analysis of DNA sequences, which showed that two species previously referred to the genus Eichleriella formed a related, but distinct grouping.