Amy Louise Acker was born and raised in Dallas, Texas, to a homemaker mother and a lawyer father.

She worked as a stage actress for several seasons, including a stint at American Players Theatre in Spring Green, Wisconsin.

Acker graduated from guest star to cast member as the show entered the final episodes in April and May 2006.

[citation needed] Also in 2005, Acker provided voice acting for the character of Huntress (Helena Rosa Bertinelli) on the animated series Justice League Unlimited.

She went on to make a guest appearance on How I Met Your Mother, in which she was reunited with Angel co-star Alexis Denisof (the real-life husband of HIMYM star Alyson Hannigan), who had a recurring role on the show.

In 2010, Acker was a series regular in the ABC drama Happy Town, portraying the character Rachel Conroy.

Acker appeared in season three of the sitcom Husbands as Claudia, Brad Kelt's ex-fiancée of who unexpectedly shows up.