Anelosimus biglebowski is a species of spider in the family Theridiidae.

All specimens known have been found in the Udzungwa Scarp Forest Reserve,[1] Mufindi District, Tanzania.

[2] Anelosimus biglebowski ranges in total length from 1.80–2.10mm in males and 1.85–2.15mm in females.

[2] The only known specimens have been collected from mid-elevation rainforest in the Udzungwa Scarp Forest Reserve, most commonly from an elevation of 1650–1730m.

Habitat range is similar to Anelosimus dude, which is also close genetically.