Princess Anna Alojza Ostrogska (1600–1654) was a Polish noblewoman and heiress, known for her great fortune, and famously pious and ascetic lifestyle.

Anna was daughter of voivode of Wołyń Price Oleksander Ostrogski h. Ostrogski, the son of voivode of Kijów (Kyiv, also Kiev) Prince Konstanty Wasyl Ostrogski h. Ostrogski and Countess Zofia Tarnowska h. Leliwa and Anna Kostka h. Dąbrowa, the daughter of Jan Kostka h. Dąbrowa and Zofia Odrowąż h. Odrowąż.

Anna Alojza married Hetman Jan Karol Chodkiewicz h. Kościesza on 28 November 1620 in Jarosław.

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