Marconi was created in part from Slott's desire to introduce a character that could cause some friction in Peter's personal life.

He found this difficult to pull off as he could not find a reason why a character like Betty Brant or Aunt May could be bigoted or racist.

[4][5] Later, Anna Maria accepts "Otto's" offer to join Parker Industries and afterwards, successfully helps him earn his doctorate.

[9] Anna Maria still continues to worry for Peter, not necessarily due to his safety, but because his absences have caused some to lose faith in the company.

Unbeknownst to Anna Maria, Sajani had infused Living Brain with the consciousness of Otto Octavius, causing the robot to act clingy around her.

Slowly, Living Brain's Otto Octavius persona becomes more active as he grows jealous of Peter and fellow subordinate Aiden Blain's closeness to Anna Maria.

[17] When Spider-Man makes it to the lab amongst the clones starting to break down, Anna Maria tells him that she's invented an inverse frequency that can fix most of the problem.

After the frequency is broadcast, Peter and Anna check the building and see that Jackal, Doctor Octopus, and Gwen Stacy have been reduced to dust.

[18] At the conclusion of the "Go Down Swinging" storyline, Anna Maria was present at Horizon University where a clean-slated Superior Octopus, under the alias of Elliot Tolliver, applies for a job.

After Superior Spider-Man defeats Terrax, Anna takes him to the medical room at Horizon University and helps him remain a hero.

[21] Anna then tries to understand why Elliot left during a ceremony, where he was being given the key to the city by the mayor for his heroic actions during the "War of the Realms" storyline.

As Max Modell and Emma Hernandez are loaded onto the ambulances, Anna Maria tells Superior Spider-Man that she will go to the hospital to keep an eye on them.