After his parents died in a plane crash, Peter lived with his Uncle Ben and Aunt May, who doted and worried endlessly about the reclusive boy suddenly placed in their midst.
In his civilian life, Peter got a job as a photographer at the Daily Bugle, working for J. Jonah Jameson (a man who passionately despises Spider-Man), and attending Empire State University as a college student.
Felicia Hardy (voiced by Jennifer Hale) and her alter-ego, Black Cat, were depicted as the first potential love interests for both Peter Parker and Spider-Man, respectively, rivaled only by Mary Jane Watson.
Felicia was the well-bred, well-to-do daughter of business woman Anastasia Hardy, and had only vague memories of her father, John Hardesky, a career jewel thief known as the Cat, who had been imprisoned for years in the top secret and guarded prison of S.H.I.E.L.D.
In the fourth season "Partners in Danger" arc, Black Cat was finally introduced, as Wilson Fisk, the Kingpin of crime (whose civilian identity regularly worked with Anastasia Hardy), learned of John Hardesky memorizing the supersoldier formula and had him abducted from the prison known as S.H.I.E.L.D., the Kingpin sent Dr. Octopus (who was blackmailing Anastasia at the time with Hardesky's criminal past) to kidnap Felicia and threatened John if he did not reveal the super soldier formula, Felicia would die.
Wanting revenge on the Kingpin, much as his late father, Matt discovered that his blindness somehow heightened his senses to superhuman levels, allowing him to navigate with ease in even the most unfamiliar environments.
Colonel Nicholas "Nick" Joseph Fury (voiced by Philip Abbott in "Day of the Chameleon", Jack Angel in later episodes), an eye patch wearing war veteran thought to have been killed in combat, became the leader of the secret police organization S.H.I.E.L.D., he and the rest of S.H.I.E.L.D.
Madame Web (voiced by Joan Lee) had a recurring role in the third and fifth seasons, giving Spider-Man cryptic yet always vital advice in his adventures and battles against his foes and eventually led him to the Beyonder.
2, she returned when the "Ultimate Battle" (analogous to the "Secret Wars") occurred, which was after Spider-Man's wife, Mary Jane's clone, had died off (due to her unstable cell structure which caused her to evaporate).
Because of the degree of censorship placed on the show, this version of Carnage was quite noticeably tamer and less violent than his appearances in the comics, merely draining life from his victims, which was later restored, rather than killing them outright.
Later in "Six Forgotten Warriors" parts 1–5, he was rescued by the rest of the Insidious Six and became a member again, but would betray them and join forces with his foster father, the Red Skull (they have no relation in the comics), and his brother, Rhienholdt Kragov, who would later become Electro.
After seeing that Venom's hatred of Spider-Man is preventing him from retrieving the device, he orders Mordo to give the symbiote's recently born offspring to madman Cletus Kasady, which creates the composite villain Carnage.
Norman Osborn appears again as Green Goblin in "I Really, Really Hate Clones", the first part of two-part series finale, in which he was working for Spider-Carnage and the Kingpin in an alternate reality alongside Hobgoblin.
When Silvermane sent Hammerhead and his men to steal the mystic Tablet of Time, which can reduce the user's age, when it was arriving in New York, Spider-Man foiled the attempt and the object was taken to Empire State University to be observed by Dr. Curt Connors.
The Hobgoblin's next appearance was in the second Green Goblin story arc, in which his identity was revealed to be Jason Philip Macendale, fiancé to Felicia Hardy and had a portal-creating machine called the Time Dilation Accelerator.
Initially he operated behind the scenes until a two-part episode where his identity was revealed to Spider-Man with the help of Daredevil, who was seeking revenge for his father's murder at Kingpin's hands.
Spider-Man discovers that Mysterio was in love with a woman named Miranda Wilson, a former actress who was disfigured and planned the entire kidnapping to swap bodies with the similar-looking Mary Jane.
In an attempt to discover Spider-Man's real identity, Jameson hires a scientist to use Dr. Connors' Neogenic Recombinator to turn Gargan into a superhero called the Scorpion.
Apparently, Spider-Man found out about this whole plan with the help of J. Jonah Jameson and rescued Robbie just when Richard and Tombstone would escape with their cellmate, also putting them back to jail.
However, when the captured Spider-Man turned out to be Flash Thompson in his own costume for a prank, Jameson fires Brock due to the humiliation of faulty journalism and has two security guards throw him out.
Afterwards, Alistair Smythe attacks Brock, Osborn and Jameson with a newly rebuilt "Black Widow" Spider-Slayer, blaming them for the supposed death of his father Spencer.
In "The Alien Costume" three-parter, Brock manages to shoot pictures of Spider-Man at a crashed space shuttle, who was there to rescue the astronauts, John Jameson and his co-pilot.
Realizing that the symbiote costume's benefits are outweighed by the negative emotions it is creating, Spider-Man uses the sound of bells in the church tower to force it to leave his body, where it bonds with Brock below.
In his first three appearances in "Ravages of Time, "Shriek of the Vulture", and "The Final Nightmare", he is an elderly man who develops strong grudge against Norman Osborn for rejecting and ridiculing his work.
Calypso (voiced by Susan Beaubian) was initially introduced as a research scientist named Dr. Mariah Crawford, who was engaged to Sergei Kravinoff before his mystic serum transformed him into Kraven the Hunter.
She is a businesswoman and a single mother as Felicia's father, a career jewel thief known as the Cat, had been imprisoned for years because he had memorized the World War II super soldier formula.
Terri Lee (voiced by Dawnn Lewis) was part of the NYPD that investigated cases that involved Spider-Man and to an extent Peter Parker, yet is unable to figure out the two are one and the same.
She had many encounters with villains Spider-Man fought like Venom, the Hobgoblin, Doctor Octopus, Morbius, the Chameleon (who was imitating Anna Watson), Tombstone, Green Goblin, the Scorpion and the Shocker.
It turned out to be Mary Jane's purse when she and Peter Parker are in the process of apartment hunting but was stopped by Spider-Man and Hobie was sent to jail for violating his parole.
Warren was able to steal a sample of Spider-Man's DNA, in a possible attempt to clone him, and contacts Alistair Smythe to have Silvermane's men repaire his underwater laboratories.