[1] Anna Maria was born at Jägerndorf, the eldest daughter of George, Margrave of Brandenburg-Ansbach and his second wife Hedwig of Münsterberg-Oels, daughter of Charles I of Münsterberg-Oels.

Anna Maria was brought up as a Lutheran and on 24 February 1544 became the wife of Christoph, Duke of Württemberg.

[2] She later acted as guardian to her son Ludwig III, Duke of Württemberg early in his reign.

After losing both her husband and her eldest son in 1568, Anna Maria fell madly in love with the young Landgrave George I of Hesse-Darmstadt (1547–1596), who was half her age and later became her son-in-law.

She died in 1589 and was buried in the St. George's Collegiate Church in Tübingen.