Located on the west side of Aqueduct Racetrack near Pitkin Avenue in the South Ozone Park neighborhood of Queens,[6] it is served by northbound A trains at all times[7] and by the northbound Rockaway Park Shuttle during summer weekends.

From 2011 to 2013, it was closed and rebuilt to provide better access to the Resorts World Casino, and an elevator was installed to make the station compliant with the Americans with Disabilities Act of 1990.

After a fire on May 8, 1950, cut service, the bankrupt LIRR sought to sell or abandon the line altogether.

[9]: 68 [12][13] In 1955, the New York Racing Association (NYRA) chose to upgrade Aqueduct Racetrack in South Ozone Park in part because of its proximity to the Rockaway Beach Branch.

[12][15] In order to allow for the switching manoeuvres required for southbound trains to stop at the station, major signal changes were made on the line.

[3][4][16] The NYRA agreed to reimburse the New York City Transit Authority (NYCTA) for annual expenses.

[3][4][16] On the start of service in September 1959, extra-fare "Aqueduct Special" (signed as S) trains ran nonstop from the lower level of the 42nd Street–Port Authority Bus Terminal in Manhattan until it crossed over to the northbound platform to discharge passengers at the racetrack.

[18][19] Transit officials said that the actual time of the specials would be thirty minutes at a minimum as the pace of the train was too swift.

[37] In October 1966, the NYRA reached its final agreement to cover the $5,000 cost of the maintenance and the operation of the station.

[32][39] In September 1978, the JFK Express began service and stopped at this station on racing days.

As part of the agreement, the northern underpass entrance at Aqueduct—North Conduit Avenue would be reopened, shortening the walking distance for people arriving at Aqueduct Racetrack by 900 feet (270 m).

The station was reopened using new MetroCard-only High Entrance/Exit Turnstiles (HEETs), and without a token booth, allowing it to be unstaffed at all times.

[57] As of July 2012, the Metropolitan Transportation Authority (MTA) has withheld approvals to construct a southbound platform.

Southbound trains run directly from Rockaway Boulevard to Aqueduct–North Conduit Avenue without stopping at Aqueduct Racetrack.

[62]: 54 The station is the only through stop in the New York City Subway to serve trains in a single direction.

[51] Resorts World Casino runs a shuttle bus between Aqueduct Racetrack and Aqueduct–North Conduit Avenue.

[65][66] Two wide staircases and the elevator lead down from platform level to the parking lots in front of the racetrack.

[57][67] A sidewalk on the western side of Aqueduct Road leads south from Aqueduct Racetrack to the North Conduit Avenue station; the main fare control area is located at the south end of that station at North Conduit Avenue.

[65] At the north end of the passageway at Pitkin Avenue is a gate which, when open, allows access between the station and racetrack and the local community.

[55][63] Formerly, the only entrance to the station was through a large wide passageway (similar to those at Mets–Willets Point), which led directly to the racetrack.

[49]: PDF p. 163  A 2014 analysis by The Wall Street Journal found that 72.6 percent of riders at the Aqueduct Racetrack station received a senior citizens' discount.