The route runs 10.30 miles (16.58 km) from Highway 9/Highway 10 south of Perryville east to Highway 60/Highway 113 in Houston.

[1] The route begins at Highway 9/Highway 10 south of Perryville.

Highway 216 runs along the southern shore of Lake Harris Brake to Antioch, where a concurrency begins with Highway 300.

The routes run north until the northeast corner of the Lake, when Highway 300 turns west, and Highway 216 continues north.

Media related to Arkansas Highway 216 at Wikimedia Commons