Arxula adeninivorans

[2] After the first identification in the Netherlands, strains of this species were later on also found in Siberia and in South Africa in soil and in wood hydrolysates.

On the one hand, it is a source for many enzymes with interesting properties and the respective genes, for instance glucoamylase, tannase, lipase, phosphatases and many others.

Here are two special examples of recombinant strains and their application: in both cases several plasmids with different foreign product genes were introduced into the yeast.

In a first case this recombinant yeast strain acquired the capability to produce natural plastics, namely PHA (polyhydroxyalkanoates).

The respective genes phbA, phbB and phbC were isolated from the bacterium Ralstonia eutropha and integrated into plasmids.

Such strains can be cultured in the presence of wastewater and the estrogens present in such samples can be easily quantified by the amount of the reporter gene product.

Design and functionality of CoMed vector system. The CoMed basic vector contains all E. coli elements for propagation in the E. coli system and a MCS (multiple cloning ste) for integration of ARS, rDNA, selection marker and expression cassette modules. For this purpose, ARS fragments are flanked by Sac II and Bcu I restriction sites, rDNA regions by Bcu I and Eco 47III restriction sites, selection markers by Eco 47III and SalI restriction sites and romoter elements by Sal I and Apa I restriction sites.