The Women's 400 metres T54 event at the 2012 Summer Paralympics took place at the London Olympic Stadium on 3 September.

[1] The event consisted of 2 heats and a final.

Typically this may be caused by a lower spinal cord injury or spinal cord birth defect.

Prior to the competition, the existing World and Paralympic records were as follows:[2] Competed 3 September 2012 from 11:18.

rule: first 3 in each heat (Q) plus the 2 fastest other times (q) qualified.