The women's 400 metres T54 event at the 2020 Summer Paralympics in Tokyo, took place on 2 September 2021.

The T54 category is for wheelchair athletes with no leg function, but near full arm function and reasonable to normal trunk function.

Typically this may be caused by a lower spinal cord injury or spinal cord birth defect.

Prior to the competition, the existing records were as follows:[1] Heat 1 took place on 2 September 2021, at 12:42:[2] Heat 2 took place on 2 September 2021, at 12:49:[3] The final took place on 2 September, at 19:40:[4]