The Borzna Regiment (Ukrainian: Борзнянський полк, romanized: Borznianskyi polk) was one the territorial-administrative subdivisions of the Cossack Hetmanate.

The regiment's capital was the city of Borzna, now in Chernihiv Oblast of northern Ukraine.

Regiment was raised by colonel Petro Zabila on territory of Borzna Volost in 1648, during Khmelnytsky Uprising.

The regiment was reactivated in 1654 as part of preparations for Invasion of the Polish–Lithuanian Commonwealth, with colonel Petro Zabila becoming commander once again.

The regiment tok part in third siege of Bykhaw in October 1654.