The Hlukhiv Regiment (Ukrainian: Глухівський полк, romanized: Hlukhivskyi polk) was one the territorial-administrative subdivisions of the Cossack Hetmanate.

The regiment's capital was the city of Hlukhiv, now in Sumy Oblast of northeastern Ukraine.

The Hlukhiv Regiment was founded in 1663 by hetman Ivan Briukhovetsky.

It was formed from sotnias of Nizhyn Regiment located on different bank of Seym river.

Заруба, Віктор (2007), Адміністративно-територіальний устрій та адміністрація Війська Запорозького у 1648-1782 рр.