Slice preparation

[2] Free hand sectioning is a type of preparation techniques where a skilled operator uses razor blade for slicing.

[3] When investigating mammalian CNS activity, slice preparation has several advantages and disadvantages when compared to in vivo study.

The physiological conditions of the sample, such as oxygen and carbon dioxide levels, or pH of the extracellular fluid can be carefully adjusted and maintained.

The slice preparation procedure itself induces a rapid and robust phenotype change in microglia, the consequences of which need to be taken into consideration when interpreting results.

Finally, the artificial composition of the bathing solution means that the presence and relative concentrations of the necessary compounds may not be present.