It maintains the world's largest collection of Burchfield's work, as well as many other distinguished artists of Buffalo, Niagara and Western New York.

[1] The museum moved to a new home located on 4.9 acres (20,000 m2) of land at the corner of Elmwood Avenue and Rockwell Road in Buffalo, New York in November 2008.

[2] Its "Front Yard" exhibit features three tall steel projectors which project constantly-changing artwork onto the exterior of the building.

Notable artists in the collection also include Claire Shuttleworth, Virginia Cuthbert, Charles Cary Rumsey, Steina Vasulka, Paul Sharits, Ruth Erb Hoffman, and many others.

It also includes work by Hallwalls founders: Charles Clough, Robert Longo, Diane Bertolo, Nancy Dwyer, Cindy Sherman, and Michael Zwack.