[3] Homologs exist as distant as the green sea turtle and chickens at approximately 60% sequence identity, suggesting that the gene may have arisen in the amniotes after their divergence from other tetrapods;[5] the first 4 exons are conserved with 36% identity as distantly as the anemone.

[8] Amino acids 229-652 contain a domain of unknown function (DUF4552) which is conserved in vertebrates.

Experimental evidence shows that C12orf40 has a physical interaction with dynein light chain 2 (DYNLL2).

[11] An analysis of normal human tissues shows that C12orf40 expression occurs primarily in the testis,[12] suggesting importance to the male reproductive system.

[14] In pigs, a region homologous to human C12orf40 plays a role in arthrogryposis, a disease characterized by congenital fibrosis.