Finally, interaction with ER is facilitated through the binding of the FFAT motif with vesicle-associated membrane protein.

[15] CERT – when expressed in mammalian cells – has been found to receive a lot of possible phosphorylations at the serine repeat (SR) motif, which is close to the PH domain.

[16] It has been shown that the phosphorylation of this SR motif leads to inactivation of the PI4P-binding and ceramide transferring activities of CERT, since it induces an autoinhibitory reaction between the PH and START domains of CERT, transforming it from the active form to the inactive form.

[19] Dephosphorylated CERT is in the active form in order to be functional and transfer ceramide from ER to Golgi.

[20] The chemically synthesized compound N-(30hydroxy-1-hydroxymethyl-3-phenylpropyl)dodecamide (HPA-12) has been found to be an inhibitor of CERT-mediated ceramide trafficking.