This process leads to a steady expansion of the phragmoplast and, concomitantly, to a continuous retargeting of Golgi-derived vesicles to the growing edge of the cell plate.
Once the cell plate reaches and fuses with the plasma membrane the phragmoplast disappears.
Our current understanding of various mechanisms involved in budding-off of Golgi vesicles, delivery and fusion of vesicles to initiate plant cell plate during cytokinesis and the synthesis of polysaccharides at the forming cell plate is very limited[1] (Figure 1).
[2] These gaps may be filled soon, as many genes that have been identified by mutations are analyzed and functions of their products are deciphered.
Double labeling experiments demonstrated that, unlike phragmoplast microtubules which are concentrated on the periphery of the forming plate, PDL is located across the whole width of the newly formed cell plate.