Charles Herbert DePuy (September 10, 1927 – March 14, 2013) was an American chemist known for his work in gas phase organic ion chemistry.

His father was an architectural engineer who supervised the construction of post office buildings throughout the southeastern United States and for a time in the US Virgin Islands.

Depuy enrolled at the University of California, Berkeley at age 16, but was drafted and served for 13 months as a hospital laboratory technician.

He enrolled in at Columbia University and began his graduate work in physical organic chemistry with William von Eggers Doering.

DePuy joined faculty at Iowa State University and rose quickly to the position of full professor.