He imported several families from Switzerland and established a production of Swiss cheese which was mainly exported to oversea markets.

His eldest brother's children drew lots for his estates with Kastrup and Charlottendal going to Charles Adolf Denis de Neergaard.

The author and educator Zakarias Nielsen [da] wrote his first poetry while working as a tutor on Charlottendal in 1863–1864.

On Charles Adolph Denis de Neergaard's death in 1903, Gyldenholm and Kastrupgård passed to his eldest son Carl.

He was married two times, first to Karen Blixen's sister Inger Benedicte Dinesen and later to Elisabeth Lina Perrochet.

In 1963, D. F. de Neergaard had to sell Charlottendal to the Danish state in connection with the establishment of Antvorskov Barracks.

230 hectares (570 acres) of land on the west side of the main road (formerly Augustendal) were sold to Idagaard, while Idagaardsfonden ceded approximately 50 hectares (120 acres) of woodland to Neergaard's Valdemarskilde as part of the transaction.