The hospital provides comprehensive pediatric specialties and subspecialties to infants, children, teens, and young adults aged 0–21[3] throughout the Wisconsin region.

The hospital features an ACS verified level I pediatric trauma center, one of two in the state.

In November 2020, Dwayne "The Rock" Johnson collaborated with Microsoft and billionaire Bill Gates to donate Xbox Series X consoles to Children's Wisconsin along with 19 other children's hospitals throughout the country.

[6][7][8] In addition to the Milwaukee location, there is a second location in Neenah, Wisconsin, on the campus of ThedaCare Regional Medical Center-Neenah which serves the Appleton, Green Bay, and Oshkosh metro areas.

[11] As of 2022, Children's Wisconsin has placed nationally in eight ranked pediatric specialties on U.S. News & World Report.