Paullini studied theology and medicine in Gdańsk, Königsberg, Rostock, Lübeck, Kiel and Copenhagen, was Magister Artium in Wittenberg and received his MD in Leiden.
Meanwhile, he accomplished study stays and courses in Cambridge, Oxford, Sweden, Norway and Iceland.Pauliini was the personal physician of the Prince-Bishop of Munster Christoph Bernhard von Galen and later of the Duke of Brunswick in Wolfenbüttel.
Paullini made extensive reference and resorted to both ancient and contemporary medical authorities and to folk medicine (sailors, farmers, common people).
His works reflected a deep learning based on the four temperaments theory and the outcome of a lengthy process of compiling the data obtained from his observation.
[3][4] He wrote a treatise (Flagellum salutis) on the advantage of the whip for curative purposes in various disorders and a handbook on the toad's therapeutic properties (Bufo juxta methodum et leges illustris Academiae Naturae curiosorum breviter descriptus).