[2] The only report of it in the chemical literature was a paper published by Marc Julia's group at the Pasteur Institute in 1965.

[1] Alexander Shulgin hypothesized that psilomethoxin could be biosynthesized by feeding Psilocybe cultures with 5-MeO-DMT, referencing a 1988 study by Jochen Gartz where transformation of DET into 4-HO-DET and 4-PO-DET was reported using such a method, with neither compounds having ever been found in nature.

[2] However, others have noted that psilomethoxin bears a close structural resemblance to known serotonergic neurotoxins such as 4,5-dihydroxytryptamine (4,5-DHT) and 4-hydroxy-5-methoxytryptamine (4-HO-5-MeO-T).

[7] A 2023 study by Usona Institute chemists tested samples of their sacrament and found no traces of psilomethoxin or its putative prodrug 5-methoxypsilocybin, but did find the usual constituents of psilocybin mushrooms such as psilocybin, baeocystin, and psilocin.

[6][7][8] Psychedelic researchers such as David E. Nichols have stated that the church's claims about psilomethoxin are "nonsensical and nonscientific" and the group has been criticized in the media.