Its southern terminus is with SR 16 and County Road 13 (CR 13) near Green Cove Springs.

CR 13 continues south to Hastings and then southeast, along old Dixie Highway to U.S. Route 1 (US 1, unsigned SR 5) near Bunnell.

SR 13 is known as San Jose Boulevard for much of its run through Duval County (coextensive with the city limits of Jacksonville).

A portion of the route that runs along the east bank of the St. Johns River, south of Jacksonville, is designated the William Bartram Scenic and Historic Highway.

North of Hastings, it travels along an overlap with Florida State Road 207 until it reaches Spuds, then branches off to the north running along the east bank of the St. Johns River until it reaches its northern terminus at SR 13 and SR 16.