[1][2][5][6][7] It is a tributary of Lake Ontario and is located in geographic Darlington Township, after which it is named.

[5] Darlington Creek begins at an unnamed field at the west edge of the local Rundle Road at an elevation of 145 metres (476 ft)[3] and heads southwest, under Durham Highway 2, then south, parallel to Ontario Highway 418.

It heads southeast, passes under the Canadian Pacific Railway main line, Ontario Highway 401, and the Canadian National Railway main lines, then turns south, and reaches its mouth at Lake Ontario.

[2][5] Groundwater discharge from the bluffs left from glacial Lake Iroquois feed the headwaters of Darlington Creek.

[9] The longest, Bowmanville Creek, to the east, has its headwaters in the Oak Ridges Moraine.