A large collection of locomotives may be viewed in the museum, such as the still-operational DRG 23 042, 01 1056, 41 024 and 44 404 steam engines, steam locomotive 4981 "Mainz", (a Prussian G 8) that was transferred from Turkey, the Austrian locomotive ÖBB 97 210, a rack railway steam engine from the Erzberg railway, the DB locomotive number 103 101-2 and the DRG E 16 08 electric engine, several DB Class V 36 diesel locomotives, a Wismar Railbus and a host of other railway vehicles, including several Culemeyer heavy trailers.
In addition, the evolution of fare tickets over the years is portrayed, from manually produced examples from the early years to the present-day computer printouts, as well as their related equipment.
An H0-model railway layout depicts Darmstadt station on the Main-Neckar line at the time the route was opened.
Special train services visit the museum several times a year, running between Darmstadt Ost and Bessunger Forst.
The vehicles are run by the German Museum Railway GmbH (DME) as the operating company.