Shark conservationist Misty Calhoun is invited to visit the sea-based facility Akhelios by pharmaceutical billionaire Carl Durant.

When they arrive, they meet Durant, as well as the facility's crew, which include shark trainers Trent Slater, Mike Shutello, and Josh Hooper, and computer technician Aaron Ellroy.

As a demonstration, Durant throws Aaron into the water, letting the sharks chase him: at the last possible second, he uses a special device to drive them away in formation.

Durant explains that he has been genetically altering the brains of five bull sharks to make them smarter, which renders Misty concerned.

Meanwhile, on the surface, a second shark pushes the motorboat into an electric control box, causing it to explode, and creating significant damage.

Misty then realizes that Bella went into labor after being dropped back into the pool, and has given birth to a series of shark pups, which previously attacked Craig.

Mike attempts to swim to the surface, but one of the sharks knocks him unconscious; Trent rescues him and Misty manages to revive him.

As a result of a lack of pressure stabilization in the room, the water from the pool pours in, and the group becomes separated, with Leslie knocked unconscious in the flooding; Durant and Daniel both end up alone.

Trent reveals to Misty that Durant has been doing the same experiments on himself using a genetics-altering liquid to make himself more intelligent, due to his phobia that technology will one day rule the world.

In 2008, Warner Premiere was planning a direct-to-video sequel to Renny Harlin's Deep Blue Sea,[5] which would be directed by Jack Perez and released sometime in 2009.

[6] Even so, in June 2017, it was announced that the sequel directed by Darin Scott had begun filming in Cape Town, South Africa.

The soundtrack was released under Dragon's Domain Records and on the back on the CD case it can be read that "A Limited Edition of 500 Units".