The route passes through many Delaware scenes, including beaches, farmland, state parks, and towns.

[2] Bike Route 1 begins at the Maryland border in the beach town of Fenwick Island.

From Bethany Beach, the bike route continues north on Delaware Route 1, passing through the Sussex Shores area before entering Delaware Seashore State Park, a state park located on an isthmus with the Atlantic Ocean to the east and the Rehoboth Bay and the Indian River Bay to the west.

The state park stretches between Sussex Shores to the south and Dewey Beach to the north.

The bike route enters a more rural, agricultural setting as it heads toward the historical shipbuilding town of Milton.

The bike route follows DE 30 to Johnson Road, which it turns west on, passing through the community of Lincoln.

Bike Route 1 then crosses a branch of the Mispillion River into Kent County, where it heads to the community of Williamsville.

This segment of the route features a bike path separate from the roadway, paralleling it to the south.

It heads north on Turkey Point Road, paralleling a Delmarva Central Railroad line, and passes through Viola before reaching the town of Woodside.

The bike route meets the western outskirts of the Delaware state capital, Dover.

The route enters New Castle County, heading north on Clayton Greenspring Road.

It then heads north on Delaware Route 71 into Middletown, running along South Broad Street.

It then turns east on Howell School Road, running along the northern boundary of Lums Pond State Park.

It then crosses Delaware Route 273 and proceeds through the Hillside Heights neighborhood on North Brownleaf Road.

It then turns to the north onto Skyline Drive and then crosses Delaware Route 7, proceeding to the northeast on Stoney Batter Road.

It then turns north on Delaware Route 100, passing by the Winterthur Museum, Garden and Library (the former home of Henry Francis du Pont) and the Brandywine Creek State Park.