[1] Deuterocharacium consists of solitary cells that are attached to a substrate via a short mucilaginous pad or a pedicel.

Deuterocharacium has been described as uninucleate (i.e. containing a single nucleus);[1] other sources state the adult cells are multinucleate,[2] but this may be before the zoospores are formed.

[1] Deuterocharacium reproduces asexually; sexual reproduction has not been observed in this genus.

Zoospores are produced in groups of 32 to 128, and are released through a tear in the mother cell wall.

[1] Within Deuterocharacium, two species are known, D. polyplastidicum and D. fallax, which differ based on the shape of the cells.