Diaphonization

[1] Diaphonization was first developed by O. Schultze in 1897, and later was modified by numerous researchers.

[2][3][4][5] Clearing renders the animals transparent and is achieved by bathing the specimens in a soup of trypsin, a digestive enzyme that slowly breaks down flesh.

The dyes alizarin red and alcian blue are most commonly used in the staining of bone and cartilage accordingly.

Advancements in imaging technology have rendered the practice all but obsolete, though it is expanding as an art form.

[6] Diaphonization is not suitable for animals longer than 30 centimeters (except for snakes) due to the limited ability of the trypsin bath to penetrate the tissues of larger animals.