Diego Gutiérrez de Humanes (1607 - 1660s) was a Spanish politician and military man who served in the Viceroyalty of Peru as Lieutenant Governor of Santa Fe, Argentina.

[1] Diego Gutiérrez de Humanes was born 1607 in Buenos Aires.

As the son of Pedro Gutiérrez and Mayor Humanés de Molina, he belonged to a distinguished Spanish family.

[4] His brother Juan Gutierrez de Humanes, served as Alcalde of second vote in Buenos Aires in 1648.

[5] Diego Gutiérrez de Humanes, and his brother had received land grants in Parana, Santa Lucía and in the present territories of San Pedro and Arrecifes.