[4] By her father's second marriage she had a brother and three sisters: After Sir Thomas Kitson's death, Dorothy's mother, Margaret, married secondly Sir Richard Long (d.1546) of Wiltshire, Great Saxham and Shingay, Cambridgeshire, Gentleman of the Privy Chamber to King Henry VIII, by whom she had a son, Henry Long, and three daughters, Jane, Katherine and Mary.
[12] Sir Thomas Pakington inherited the lordship of Aylesbury, Buckinghamshire, from his maternal grandfather, John Baldwin, Chief Justice of the Common Pleas.
[12] Lady Dorothy's eldest son, Sir John Pakington, was for a time a favourite of Queen Elizabeth I, who invited him to court after he had been presented to her during her visit to Worcester in 1572.
[14] In addition to being one of the few widows to nominate Members of Parliament, Lady Dorothy was also among the few women of the Tudor period to make a last will and testament while her husband was still living.
[15][16] Dorothy Kitson married firstly Sir Thomas Pakington (died 2 June 1571) of Hampton Lovett, Worcestershire.