Drosophila busckii is a species of fruit fly that is native to North America, though it now also occurs in Asia, Europe, Oceania and South America.

[1] It can be identified by the presence of dark stripes on the thorax (including a trident shape on the mesonotum) and the wings being transparent with no markings.

[3] In the laboratory they are normally reared on Wheeler-Clayton food.

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