Both ESC and EpiSC induce teratoma when injected in the test animals (scid mice) which proves pluripotency.
One of those methods is overexpressing in the primed pluripotent stem cell a reprogramming factor Klf4,[4] or Sox2 and Klf4 (SK cocktail).
Moreover, beyond the potency-state comparison, MLL1 inhibition was also shown to reactivate the silenced X-chromosome which is typically deactivated in post-implantation epiblast stem cells, suggesting an epigenetic reversion back to a more ground-level, naive state.
[7] Many studies have used EpiLCs as suitable analogues for actual post-implantation derived epiblast stem cells, especially in attempts at reversion back to the "naive" state.
Recently, overexpression of PR-domain Zinc Finger Protein 14 (PRDM14) in EpiLC was shown to cause a reversion back to an ESC-like state (with levels of Alkaline Phosphatase staining recovered to that observed in ESCs as well as more ESC-like cell morphology), with Klf2 being required for the mechanism to occur.