Jason Haynes (Jules Robertson) visits Jac Naylor (Rosie Marcel) and Alexandra 'Lexy' Dunblane (Jenny Howe) on the Darwin ward.
Nicky McKendrick (Belinda Owusu) and Eli Ebrahimi (Davood Ghadami) carry out successful heart surgery on a patient.
Henrik Hanssen (Guy Henry), Donna Jackson (Jaye Jacobs) and Jeong-Soo Han (Chan Woo Lim) work on Keller ward where they care for Lexy, who is in need of a kidney and heart transplant.
Dominic Copeland (David Ames), Ange Godard (Dawn Steele), Josh Hudson (Trieve Blackwood-Cambridge), Madge Britton (Clare Burt), Kylie Maddon (Amy Murphy) and Louis McGerry (Tyler Luke Cunningham) work the shift on AAU ward as they seek a diagnosis for patient Ken Davies (Hamish Clarke).
Dominic and Ange argue over Josh's bulimia secret but are forced to work together in an operating theatre to save Ken's life.
With Adrian "Fletch" Fletcher (Alex Walkinshaw) as her witness, Jac makes an advance directive to request no medical intervention in the event of further complications.
Joseph Byrne (Luke Roberts), Ric Griffin (Hugh Quarshie) Serena Campbell (Catherine Russell) and Bernie Wolfe (Jemma Redgrave) all receive Jac's organs at various locations to perform transplant procedures.
All medical staff on the three main wards are notified about a major incident and they rush outside to attend the emergency; despite not being given time to adjust to their grief.
Story producer Ben Wadey stated that theme was "to show the resilience in characters who work there all of the time, they give their lives to the profession.
In November 2021, Rosie Marcel who plays Jac Naylor told Laura-Jayne Tyler from Inside Soap that the final episode would be "spectacular" and "amazing".
[12] Inside Soap's Tyler revealed that the episode would focus on the immediate aftermath of Jac's failed surgery to remove a terminal brain tumour.
[12] Holby City's story producer Ben Wadey did not want to leave viewers feeling "frustration" that the show had "unfinished business".
Ange shares her final scene on the AAU ward with her fiancée Josh Hudson (Trieve Blackwood-Cambridge) and her son Dominic, where they are all at peace with one another.
He teased "a twinkle of happiness" for the character, referring to Henrik going off to be with his love interest Russell "Russ" Faber (Simon Slater).
"[8] In addition, writers created other smaller stories such as Nicky McKendrick (Belinda Owusu) performing surgery on a beating heart and an ending for the recurring character Alexandra 'Lexy' Dunblane (Jenny Howe).
"[15] Producers decided to bring back various characters for the episode, which included Joseph Byrne (Luke Roberts), Mo Effanga (Chizzy Akudolu), Ric Griffin (Hugh Quarshie) Serena Campbell (Catherine Russell) and Bernie Wolfe (Jemma Redgrave).
[17] Ames told Calli Kitson from Metro that "the hospital must go on" to represent the NHS and "to show that while other people's lives have dramas going on they still do their job and they still keep going, and that's what we wanted to depict.
Belinda Owusu (Nicky McKendrick) felt lucky to be working on the episode and Ames stated that "emotions are running high but we have pretend lives to save.
[8] In the build up to the episode's broadcast, cast members such as Rosie Marcel and Alex Walkinshaw gave interviews with press to promote the show's end.
[29] Gemma Jones of the Liverpool Echo branded Jac's death as "emotional" to the point it left fans "heartbroken" and in tears.
Denby concluded that producers were correct to kill Jac off because a series finale episode needs a "game-changing narrative" to create a "big farewell".
[37] Their colleague Victoria Wilson, this time writing for Whattowatch.com said that various returning characters gave the show a "fitting finale" for a continuing drama.
[39] Sue Haasler from Metro praised the episode and the inclusion of "humour in among all the tragedy" as characters recount their favourite insults that Jac made.
"[41] Dainty concluded that the episode contained "subtle irony to overt symbolism" and overall it was "a celebration" of the NHS, Jac Naylor, Holby City and its fans.
She said that unlike Brookside and Eldorado, the show gave a final episode "far more dignified" by "going out gracefully" with a "beautifully and subtly played goodbye.
"[42] Judith Woods from The Daily Telegraph branded it a "beautifully-judged finale", "the most powerful encomium" and "the most poignant love letter to the NHS".
"[45] Alice Dear from Heart radio opined that the episode "paid tribute to the NHS" and stated that viewers applauded the writers, cast and the production team.
Cook thought Jac's death was the "tragic centrepiece of the episode" and Henrik and Russ' scene a "touching final reunion".
[49] Cathy Owen from Wales Online believed "it was emotional for different reasons" including Jac's death alongside Max and Sacha's reactions.
[50] Jessica Sansome of Manchester Evening News believed that the episode was effective in showing the possible lives that organ donation can save.