Essen-Holthausen station is located in the district of Überruhr in the city of Essen in the German state of North Rhine-Westphalia.

A bridge structure connects these two side platforms to Langenberger Straße just over 100 metre to the east, where the Holthausen Bf stop of the Ruhrbahn is located.

The station was opened in 1945 to serve the Heinrich colliery, but was closed again a year later.

The station is served by Rhine-Ruhr S-Bahn line S 9 (Recklinghausen / Haltern – Gladbeck - Essen - Wuppertal - Hagen), operating every 30 minutes during the day.

These serve the Holthausen Bf stop located more than 100 metres east, but on the level of the station.