First Presbyterian Church is a historic Presbyterian church located in the Fisher Park Historic District of Greensboro, North Carolina.

Thirty to 40 slaves were members by the time of the American Civil War, and after being freed, 37 former slaves started Saint James Presbyterian at Friendly Avenue and Church Street.

The first three were located at Church Street and Summit Avenue north of the city center.

The third building on that site, a Romanesque Revival style brick structure, now houses the Greensboro Historical Museum.

In 1929 First Presbyterian Church moved into its fourth and current building, a Gothic Revival cathedral overlooking Fisher Park.